Methylation Products:
Methylation Cancer Panel I: Based on Illumina's Golden Gate Chemistry, this methylation product assays 1,505 CpG loci selected from 807 genes. In this method, oligonucleotide primers query putative methylation sites in bisulfite-converted genomic DNA. Bisulfite treatment of unmethylated CpG converts cytosine to uracil, while methylated cytosines are protected from conversion. Similar to methylation-specific PCR, two primer sets are designed for each CpG target; one primer set corresponds to the unmethylated, bisulfite-converted sequence (uracil) while the other corresponds to the unconverted sequence (5-methyl cytosine). The extent of methylation at a given CpG site is determined by comparing the proportion of signal from methylated and unmethylated alleles in the DNA sample. Enzymatic discrimination takes place on the single nucleotide level with additional inferred methylation information for adjacent CpG sites that fall within the assay probe target region. All of the genes in this panel are also represented on the Human-G v2 Expression BeadChip. To see which genes are covered, click here (.pdf). To see a list of CpG islands probed, click here (.xls). To see a detailed list of genes and the locations probed within the genes, click here (.csv).
Custom Methylation Products: Custom Methylation Products will be available soon. Check back for more information.
Analysis software: If you wish to use Illumina's Bead Studio Methylation module to analyze your data, please contact Illumina directly (stking@illumina.com). The methylation module is not included in our Bead Studio license.